Workflow Features
- Ability to discover novel small RNA molecules and isoforms
- Sensitivity to detect small RNAs present at less than one copy per cell
- Orientation information is preserved for strand-specific expression analysis
- Cost-effective analysis of multiple samples in a single run using barcodes
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How It Works
Small RNAs are isolated from the sample and reverse transcribed to make a cDNA library of the small RNA population. The cDNA in the library is then amplified and sequenced, generating sequence fragments, commonly called "tags", corresponding to the cDNA of each small RNA. The tags are then mapped back to reference sequence databases to identify both new and known small RNA species. Relative expression levels can be calculated based on the number of tags obtained for each small RNA.
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