Workflow Features
- Ideal for quantitating individual miRNAs from a broad assortment of species, including human, mouse, rat, Drosophila, C. elegans, and Arabidopsis
- Each kit includes the TaqMan Assay and reverse transcription primer specific for the mature miRNA target of interest
- Assays are continuously added for human, mouse, and rat species to stay aligned with the Sanger miRBase Registry
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How It Works
TaqMan MicroRNA Assays employ an innovative target-specific stem-loop reverse transcription primer to address the challenge of the short length of mature miRNA. The primer extends the 3' end of the target to produce a template that can be used in standard TaqMan real-time PCR. Also, the stem-loop structure in the tail of the primer confers a key advantage to these assays: specific detection of the mature, biologically active miRNA.
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