Workflow Features
- Results the same day—Complete an experiment profiling hundreds of miRNAs in as little as 5 hours
- Ideal for human, mouse, and rat profiling
- Requires only minute sample amounts—As little as 1 ng of total RNA input—making it suitable for FFPE, FACS, biopsy, and other very small samples
- Comprehensive coverage of known miRNAs consistent with Sanger miRBase v10
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How It Works
Up to 381 miRNAs are reverse transcribed in a single reaction using Megaplex™ RT Primers, a mixture of miRNA-specific stem-loop primers. Next, an optional amplification step can be performed using Megaplex PreAmp Primers. This unbiased amplification step significantly increases the concentration of miRNAs in the sample for maximum sensitivity and detection using real-time PCR. For the final quantitation step, TaqMan® Universal PCR Master Mix is added to each sample and the mixtures are pipetted into the sample loading ports of a TaqMan Array—a pre-configured microfluidic card containing 384 TaqMan MicroRNA Assays. The real-time PCR is run on the Applied Biosystems 7900HT Fast Real-Time PCR System.
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